Wei Cheng
Postdoctoral researcher in Prof. Carlos Bustamante
Laboratory
Dept. of Molecular & Cell
Biology
209 Hildebrand Hall
E-Mail: cheng@alice.berkeley.edu
Education:
·
1991-1995 B.S.,
Biophysics, University of Science & Technology of
·
1996-2002 Ph.D.
Molecular Biophysics,
Research:
·
B.S. Molecular
dynamics simulations of a model ion channel, calculation of electrostatics
using a modified finite difference Poisson-Boltzmann
method, with Prof. Cunxin Wang and Yunyu Shi.
Research
Article
W. Cheng, C. Wang, W. Chen, Y. Xu
& Y. Shi, “Investigating the Dielectric Effects of Channel Pore Water on
the Electrostatic Barriers of the Permeation Ion by the Finite Difference
Poisson-Boltzmann Method”, Eur.
Biophys. J. (1998) 27: 105-112. [Abstract]
[PDF]
·
Graduate lab
rotations: kinetics of DNA helicase binding to
single-stranded DNA with Prof. Tim Lohman; computer
modeling of the packing of transmembrane a-helices with Prof. Garland Marshall; substrate
specificity of human a-thrombin with Prof. Enrico Di Cera.
Conference
abstracts
W. Cheng & E. Di Cera, “Role of P2 Site in Substrate Recognition by
Thrombin”, 11th Annual Gibbs Conference on Biothermodynamics,
pp. 58,
W. Cheng, D. R. Lowis & G. R.
Marshall, “Amino Acid Sidechain Mapping of Transmembrane Helices in Molecular Docking”, The Protein
Society 12th Symposium, San Diego, 1998.
·
Ph.D.
Dissertation: Mechanisms of duplex DNA unwinding by E. coli Rep Helicase and a RepD2B mutant, with Prof. Timothy Lohman.
Research
Article
W. Cheng, J. Hsieh, K. M. Brendza & T. M. Lohman, “E. coli Rep Oligomers are Required to Initiate DNA Unwinding in vitro”, J. Mol. Biol. (2001) 310: 327-350. [Abstract] [PDF] [Excerpt from Reviewers’ comment]
T.
Ha, I. Rasnik, W.
Cheng, H. P. Babcock, G. H. Gauss, T. M. Lohman
& S. Chu, “Initiation and Re-initiation of DNA Unwinding by the E.
coli Rep Helicase”, Nature (2002) 419:
638-641. [Abstract]
[PDF]
[Supplementary
Information]
W. Cheng, K. M. Brendza, G. H. Gauss, S. Korolev, G. Waksman & T. M. Lohman, “The 2B Domain of E. coli DNA Helicase Rep is not Required for Duplex DNA Unwinding Activity”, Proc. Natl. Acad. Sci., USA. (2002) 99: 16006-16011. [Abstract] [PDF] [Supporting Information] [Excerpt from Reviewers’ comment]
I. Rasnik, S. Myong, W. Cheng, T. M. Lohman
& T. Ha, "DNA-binding Orientation and Domain Conformation of the
E.
coli Rep Helicase Monomer bound to a Partial
Duplex
Junction: Single-molecule Studies of Fluorescently Labeled Enzymes",
J. Mol. Biol. (2004) 336: 395-408.
[Abstract]
[PDF]
M.C. Murphy, I.
Rasnik, W.
Cheng,
T.M. Lohman & T. Ha, "Probing single
stranded DNA conformational flexibility using fluorescence
spectroscopy", Biophys. J. (2004)
86:
2530-2537. [Abstract]
[PDF]
K.
M. Brendza*, W.
Cheng*, C. J. Fischer, M. A. Chesnik, A. Niedziela-Majka, and T. M. Lohman,
“Auto-inhibition of E. coli Rep Monomer Helicase
Activity by its 2B-Subdomain”, Proc. Natl. Acad. Sci.,
Book
Chapter
T.
M. Lohman, J. Hsieh, N. K. Maluf,
W. Cheng, A. L. Lucius,
C. J. Fischer, K. M. Brendza, S. Korolev
& G. Waksman, “DNA Helicases,
Motors that Move Along Nucleic Acids: Lessons from the SF1 Helicase
Superfamily”, In THE
ENZYMES, 3rd Edition, edited by D. D. Hackney & F.
Tamanoi, Volume XXIII, Energy Coupling and Molecular
Motors, (2004) 304-364.
·
Postdoc. Single-molecule studies of Hepatitis C virus NS3 helicase, with Prof. Carlos Bustamante and in collaboration with Prof. Anna Marie Pyle at Yale University.
Research
article
S. Dumont*,
W. Cheng*, V. Serebrov, R. K. Beran,
I. Tinoco Jr., A. M. Pyle, & C. Bustamante, “Direct Observation of Substeps
Reveals the RNA Translocation and Unwinding Mechanism of HCV NS3 Helicase and its Coordination by ATP”, Nature (2006) 439: 105-108. [Abstract]
[PDF]
[Supplementary
Information] *Equal authorship.
Last Updated
01/08/06.